This sweet potato may not only decrease fasting blood glucose and LDL (bad) cholesterol levels but also increase insulin sensitivity ( 43, 44, 45). Given the relatively high GI of sweet potatoes, large amounts in a single meal may be unsuitable for people with type 2 diabetes. Notably, boiling seems to be associated with lower GI values than baking, frying, or roasting ( 7). Starch Cornelio MTMN, Figueiroa ARS, Santos KGB, Carvalho R, Filho WSS, Guerra M (2003) Chromosomal relationships among cultivars of Citrus reticulata Blanco, its hybrids and related species. Plant Syst Evol 240:149–161 Ravi M, Chan SW (2010) Haploid plants produced by centromere-mediated genome elimination. Nature 464:615–618

Generally, the evolutionary data of WGD events were estimated by Ks values ( Wang et al., 2011; Tiley et al., 2018; Zwaenepoel and Van De Peer, 2019). As shown in Table 1, we found that the Ks values of CsSWEET duplication pairs ranged from 0.0103 to 2.1158 ( Supplementary Table 1 and Supplementary Figure 3). Subsequently, we found that CsSWEET3c/CsSWEET3a, CsSWEET3a/CsSWEET3b, and CsSWEET2b/CsSWEET2a might be generated through a recent WGD event, while CsSWEET3c/CsSWEET3b and CsSWEET10c/CsSWEET12 might be generated through an ancient WGD event, suggesting that these two rounds of WGD events might play key roles in the expansion for CsSWEET genes in C. sinensis. The Expression of CsSWEETs Reveals Possible Functions in C. sinensis Soluble fibers, such as pectin, may increase fullness, decrease food intake, and reduce blood sugar spikes by slowing your digestion of sugars and starches ( 15, 16). ANDERSON: Constantly. It was cool at the Live Events last weekend, when we came out, everybody in the crowd was Too Sweeting. It got me pumped up, man. It’s everywhere. BÁLOR: Originally, it was just me and Karl [in Japan]. Then as Gallows came in, we started Too Sweeting him. It was kind of like an inner circle deal. Now, when people see me, they’ll throw up a Too Sweet. In the beginning, I was reluctant to give it to people who weren’t in the inner circle. But now, I’m embracing it left, right and center.Simple sugars, such as glucose, fructose, sucrose, and maltose, comprise 32% of the carb content ( 2).

Those moments when everyone’s happy – Teddie’s crawling around, Millie’s running around – and it’s all easy-going,’ she says.Camellia sinensis, which originated from the tropical area of Southwest China, is an important economic beverage crop in China ( Wei et al., 2018; Zhang et al., 2020b). Sugar transport and homeostasis contribute to plant growth and development ( May et al., 1998; Lastdrager et al., 2014; Rahimi et al., 2019; Pan et al., 2020; Saddhe et al., 2020). Some published papers focused on the genes that participated in sugar metabolism, such as hexose kinase, invertase, and galactinol synthase, in C. sinensis ( Yue et al., 2015; Zhou et al., 2017; Samarina et al., 2020). Due to the economic interest in C. sinensis as a beverage crop, its genome, proteome, and transcriptome were recently sequenced and released ( Wei et al., 2018; Wu et al., 2018; Liu et al., 2020; Xia et al., 2020; Zhang et al., 2020b), which help us to analyze the SWEET genes in C. sinensis systematically. In the present study, 26 CsSWEETs in C. sinensis were identified, and their gene structures, phylogenetic, microsynteny, and expression were analyzed. Our study revealed the functions of these CsSWEETs and provided candidate genes for further research. Materials and Methods Database Search for C. sinensis Genome NASH: Everyone calls it the Too Sweet because I used to throw it up in The nWo and call it “Too Sweet,” but it was already established long before The nWo was around.

According to the predicted sequences and GFF annotation files, we determined the distribution of exon–intron of the 26 CsSWEET genes in C. sinensis. As shown in Supplementary Figure 1, most CsSWEET genes contained five introns; however, several of the members from the CsSWEET gene family contained two, three, or four introns. Remarkably, there are extreme differences in the number of introns from the CsSWEET subfamily II, ranging from 4 to 14, indicating that these CsSWEETs may have experienced intron loss or gain some during evolution ( Supplementary Figure 1). These results were also confirmed by previous studies. For example, Cao et al. (2019) found that the number of introns from VfSWEETs ranged from four to 10. Additionally, we found that, although the lengths of members from CsSWEET gene family vary, introns stepped into almost the same position of the gene open reading frame. Deng XX, Deng ZA, Xiao SY, Zhang WC (1992) Pollen derived plantlets from anther culture of chang papeda hybrid No. 14 and trifoliate orange. In: Proceedings of the 7th international citrus congress, acireale, Italy, March 1992. International society of citriculture, pp 190–192 So it was outside of my field of reference until I was well into my transition. I was happy with who I was and where my destiny was in the world.’Deficiency can cause both temporary and permanent damage to your eyes and even lead to blindness. It can also suppress immune function and increase mortality, especially among children and pregnant and lactating women ( 14, 40).

The intensity of the yellow or orange color of the sweet potato is directly linked to its beta carotene content ( 41). Starches are often split into three categories based on how well they’re digested. The starch proportions in sweet potatoes are as follows ( 8, 9, 10, 11): Slowly digested starch (9%). This type breaks down more slowly and causes a smaller rise in blood sugar levels. ANDERSON: We loved The nWo. We loved Scott Hall. We loved Kevin Nash. It was just kind of our thing.Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424 In the present study, we identified 28 CsSWEETs in the C. sinensis genome. Further analyses for ML tree, intron–exon, and duplication suggested that the conservation of CsSWEETs was accompanied by a certain degree of divergence. The expression profiles of all CsSWEETs suggested that several genes were highly expressed in reproductive tissues, indicating that these genes played important roles in sugar transport. Additionally, 28 CsSWEETs were differently expressed for RNA-seq data under cold and drought stresses, indicating that tea plants might have developed specialized regulatory mechanisms for different abiotic stresses. Data Availability Statement The MAFFT (version 7) software was used to execute the multiple alignments of all SWEET proteins with default parameters ( Katoh et al., 2005). The best substitution model of these SWEET proteins was determined by ModelFinder software ( Kalyaanamoorthy et al., 2017). The maximum likelihood (ML) tree was determined by IQ-tree (version 2.1.2) software ( Nguyen et al., 2015) with an SH-aLRT test for 1,000 random addition replicates and a bootstrap test for 10,000 replicates as described by Cao et al. (2020a). The Figtree software was used to visualize this ML tree. Internal Repeats and Topological Analyses The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fgene.2021.655843/full#supplementary-material