The user has license keys but they are not valid. Reasons for the invalid licensing keys might be due to the following: Liang, T. et al. UVR8 interacts with BES1 and BIM1 to regulate transcription and photomorphogenesis in Arabidopsis. Dev. Cell 44, 512–523 (2018).

Graindorge, S., Cognat, V., Berens, P. J., Mutterer, J. & Molinier, J. Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure. PLoS Genetics 15, e1008476 (2019). Li, C. F. et al. Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. PLoS Genet. 4, e27 (2008). You can choose to place only your key users in the OU you specify for this solution. In this way you can have a reduced cost and keep your most important users always productive at the same time. The following architecture shows the DR activation process in case the production region becomes unavailable. Gao, Z. et al. An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation. Nature 465, 106–109 (2010).Johnson LM, Law JA, Khattar A, Henderson IR, Jacobsen SE (2008) SRA-domain proteins required for DRM2-mediated de novo DNA methylation. PLoS Genet 4: e1000280. Tsukahara S, Kobayashi A, Kawabe A, Mathieu O, Miura A, et al. (2009) Bursts of retrotransposition reproduced in Arabidopsis. Nature 461: 423–426. Lister R, O'Malley RC, Tonti-Filippini J, Gregory BD, Berry CC, et al. (2008) Highly integrated single-base resolution maps of the epigenome in Arabidopsis. Cell 133: 523–536. Greco, M., Chiappetta, A., Bruno, L. & Bitonti, M. B. In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning. J. Exp. Bot. 63, 695–709 (2012).

Gowher H, Liebert K, Hermann A, Xu G, Jeltsch A (2005) Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L. J Biol Chem 280: 13341–13348. A synthetic DNA fragment encoding the methyltransferase domain of A. thaliana DRM2 (residues 270 to 626) was cloned into pRSFDuet-1 vector (Novagen), preceded by an N-terminal His 6-SUMO tag. The expression plasmid was transformed into Escherichia coli BL21 DE3 (RIL) cells, and the cells were grown at 37°C. After the cell density reached an optical density at 600 nm of 0.8, the temperature was lowered to 16°C. Subsequently, the cells were induced by 100 μM isopropyl-β-D-thiogalactopyranoside and continued to grow overnight. The cells were collected and resuspended in lysis buffer [50 mM tris-HCl (pH 8.0), 1 M NaCl, 25 mM imidazole, and 1 mM phenylmethylsulfonyl fluoride] and lysed using an Avestin Emulsiflex C3 homogenizer. After centrifugation, the supernatant was applied to a Ni 2+–nitrilotriacetic acid affinity column and the His 6-SUMO-DRM2 fusion protein was eluted with elution buffer [20 mM tris-HCl (pH 8.0), 300 mM NaCl, and 300 mM imidazole]. The His 6-SUMO tag was then removed by ubiquitin-like protease 1–mediated cleavage. The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare). The final protein sample was concentrated and stored in −80°C freezer for future use. Li CF, Henderson IR, Song L, Fedoroff N, Lagrange T, et al. (2008) Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. PLoS Genet 4: e27. Henderson IR, Jacobsen SE (2008) Tandem repeats upstream of the Arabidopsis endogene SDC recruit non-CG DNA methylation and initiate siRNA spreading. Genes Dev 22: 1597–1606.

Zientara-Rytter, K. & Sirko, A. Significant role of PB1 and UBA domains in multimerization of Joka2, a selective autophagy cargo receptor from tobacco. Front. Plant Sci. 5, 13 (2014). Bourc'his D, Xu GL, Lin CS, Bollman B, Bestor TH (2001) Dnmt3L and the establishment of maternal genomic imprints. Science 294: 2536–2539. Initiate the license verification by calling verifyLicense(). This method is exposed in the LicensingService class. It takes two parameters as input: Zhong, X. et al. DOMAINS REARRANGED METHYLTRANSFERASE3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc. Natl Acad. Sci. USA 112, 911–916 (2015). Figure 1. A conserved clade of catalytically mutated DRM cytosine methyltransferase paralogs in angiosperms.

An AWS Lambda function, triggered once a new CSV file is uploaded. It will read the CSV file containing the AD user list, and compare with the current WorkSpaces instances. If a user that exists in the AD user list does not have a WorkSpace, it will create one for it. In case a WorkSpace is created for a user that does not exist in the AD user list, it will be terminated. In this way it will keep WorkSpaces only for active users. Eun, C. et al. AGO6 functions in RNA-mediated transcriptional gene silencing in shoot and root meristems in Arabidopsis thaliana. PLoS ONE 6, e25730 (2011). Yin, R., Arongaus, A. B., Binkert, M. & Ulm, R. Two distinct domains of the UVR8 photoreceptor interact with COP1 to initiate UV-B signaling in Arabidopsis. Plant Cell 27, 202–213 (2015). Pontier D, Yahubyan G, Vega D, Bulski A, Saez-Vasquez J, et al. (2005) Reinforcement of silencing at transposons and highly repeated sequences requires the concerted action of two distinct RNA polymerases IV in Arabidopsis. Genes Dev 19: 2030–2040.Springer, N. M. & Schmitz, R. J. Exploiting induced and natural epigenetic variation for crop improvement. Nat. Rev. Genet. 18, 563–575 (2017). If you use AD Connector, as long as you have at least one active WorkSpaces attached to small directories or at least 100 active users for large directories each month, AD connector will have no additional cost. Kim, G. et al. Herbicide injury induces DNA methylome alterations in Arabidopsis. PeerJ 5, e3560 (2017).